The technology of LigandTracer is based on repeated differential measurements of surface associated proteins. Cells are seeded in a local part of a cell dish and the opposite side is used as a reference. The dish is placed on an inclined, slowly rotating support and liquid containing labeled protein is added. The detector, which is mounted over the upper part, registers the additional activity of proteins bound to the cells. Each revolution results in a peak, which is an accurate measurement of the amount of bound protein. The peak height is followed over time, providing an accurate estimation of the kinetics of the interaction.

Main benefits of LigandTracer compared to manual measurements:
  • Protein-cell interactions can be followed in real-time during minutes, hours, or days
  • Binders with rapid dissociation rates can be correctly characterized
  • Washing steps are not needed
  • Counting cells are not needed
  • LigandTracer saves reagents and labor time



Downloads

LigandTracer Technology
Protocol: A typical LigandTracer measurement
Watch video: How to run a LigandTracer experiment
Watch video: How to install your LigandTracer
Watch video: Affinity, on-rate, off-rate
Watch video: Curvature: a needed feature of binding traces